5UI0

Crystal Structure of the Tyrosine Kinase Domain of FGF Receptor 2 harboring an E565A/K659M Double Gain-of-Function Mutation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Fibroblast growth factor receptor 2	polymer	324	37054.6	2	UniProt (P21802) Pfam (PF07714) In PDB	Homo sapiens (Human)	FGFR-2,K-sam,KGFR,Keratinocyte growth factor receptor
2	A, B	CITRATE ANION	non-polymer		189.1	2	Chemie (FLC)
3	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	B	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
5		water	water		18.0	258	Chemie (HOH)

Sequence modifications

A, B: 458 - 768 (UniProt: P21802)

PDB	External Database	Details
Met 445	-	initiating methionine
Gly 446	-	expression tag
Ser 447	-	expression tag
Ser 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
Ser 455	-	expression tag
Gln 456	-	expression tag
Asp 457	-	expression tag
Ala 491	Cys 374	engineered mutation
Ala 565	Glu 448	engineered mutation
Met 659	Lys 542	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74109.1
Non-Polymers*	Number of molecules	4
	Total formula weight	979.5
All*	Total formula weight	75088.6

*Water molecules are not included.