5UCW

Cytochrome P411 P-4 A82L A78V F263L amination catalyst

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	NADPH-cytochrome P450 reductase 102A1V3	polymer	472	54006.6	2	UniProt (F2Q7T0) Pfam (PF00067) UniProt (by SIFTS) (P14779) In PDB	Bacillus megaterium
2	A, B	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3		water	water		18.0	377	Chemie (HOH)

Sequence modifications

A, B: 0 - 463 (UniProt: F2Q7T0)

PDB	External Database	Details
Leu 82	Ala 83	engineered mutation
Ala 87	Phe 88	engineered mutation
Ser 142	Pro 143	engineered mutation
Ile 175	Thr 176	engineered mutation
Val 184	Ala 185	engineered mutation
Arg 226	Ser 227	engineered mutation
Gln 236	His 237	engineered mutation
Gly 252	Glu 253	engineered mutation
Leu 263	Ile 264	engineered mutation
Gly 268	Thr 269	engineered mutation
Val 290	Ala 291	engineered mutation
Val 328	Ala 329	engineered mutation
Val 353	Leu 354	engineered mutation
Val 366	Ile 367	engineered mutation
Ser 400	Cys 401	engineered mutation
Ser 438	Thr 439	engineered mutation
Lys 442	Glu 443	engineered mutation
Leu 464	-	expression tag
Glu 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	108013.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1233.0
All*	Total formula weight	109246.2

*Water molecules are not included.