5UAG

Escherichia coli RNA polymerase mutant - RpoB D516V

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H	DNA-directed RNA polymerase subunit alpha	polymer	320	35591.6	4	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, I	DNA-directed RNA polymerase subunit beta	polymer	1342	150804.9	2	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, J	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	2	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, K	DNA-directed RNA polymerase subunit omega	polymer	90	10118.4	2	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, L	RNA polymerase sigma factor RpoD	polymer	613	70352.2	2	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli (strain K12)	Sigma-70
6	D, I, J	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	D, J	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

C, I: 1 - 1342 (UniProt: P0A8V2)

PDB	External Database	Details
Val 516	Asp 516	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	915651.2
Non-Polymers*	Number of molecules	8
	Total formula weight	358.9
All*	Total formula weight	916010.0

*Water molecules are not included.