5U2R

PRECATALYTIC TERNARY COMPLEX OF HUMAN DNA POLYMERASE BETA WITH GAPPED DNA SUBSTARTE, INCOMING L-DCTP AND CA2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase beta	polymer	343	39258.8	1	UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (Human)
2	T	16- MER TEMPLATE	polymer	16	4869.2	1		synthetic construct
3	P	10- MER PRIMER	polymer	10	3061.0	1		synthetic construct
4	D	5-MER PHOSPHORYLATED DOWNSTREAM PRIMER	polymer	5	1536.0	1		synthetic construct
5	A	4-amino-1-{2-deoxy-5-O-[(R)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}phosphoryl]-beta-L-erythro-pentofuranosyl}pyrimidin-2(1H)-one	non-polymer		467.2	1	Chemie (1S0)
6	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
7	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
8	A	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
9		water	water		18.0	286	Chemie (HOH)

Sequence modifications

A: 1 - 335 (UniProt: P06746)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	48725.0
Non-Polymers*	Number of molecules	8
	Total formula weight	770.4
All*	Total formula weight	49495.4

*Water molecules are not included.