5U21

X-ray structure of the WlaRF aminotransferase from Campylobacter jejuni, K184A mutant in complex with TDP-Qui3N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Putative aminotransferase	polymer	382	43986.4	4	UniProt (Q9ALS9) Pfam (PF01041)	Campylobacter jejuni
2	E, M (A, C)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
3	F, K, N, O (A, B, C, D)	(2R,3R,4S,5S,6R)-3,5-dihydroxy-4-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}-6-methyltetrahydro-2H-pyran-2-yl [(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl dihydrogen diphosphate	non-polymer		776.5	4	Chemie (TQP)
4	G, H, I, L, P... (A, B, D)	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
5	J (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	R, S, T, U (A, B, C, D)	water	water		18.0	1594	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 360 (UniProt: Q9ALS9)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Arg -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Ala 184	Lys 184	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	175945.7
Non-Polymers*	Number of molecules	13
	Total formula weight	3559.7
All*	Total formula weight	179505.4

*Water molecules are not included.