5TXP

STRUCTURE OF Q151M complex (A62V, V75I, F77L, F116Y, Q151M) mutant HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DDATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT	polymer	556	64049.5	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Pr160Gag-Pol
2	B, D	HIV-1 REVERSE TRANSCRIPTASE P61 SUBUNIT	polymer	428	50039.5	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)	Pr160Gag-Pol
3	T, E	DNA (5'-D(*TP*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*G)-3')	polymer	27	8383.4	2		Human immunodeficiency virus 1
4	P, F	DNA (5'-D(*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*G)-3')	polymer	21	6490.3	2		Human immunodeficiency virus 1
5	G, H	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)		sucrose
6	A, C	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
7	A, C	2',3'-dideoxyadenosine triphosphate	non-polymer		475.2	2	Chemie (DDS)
8	B	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
9	B, T, D	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
10		water	water		18.0	101	Chemie (HOH)

Sequence modifications

A, C: 1 - 554 (UniProt: P03366)

PDB	External Database	Details
Met -1	-	initiating methionine
Val 0	-	expression tag
Val 62	Ala 661	engineered mutation
Ile 75	Val 674	engineered mutation
Leu 77	Phe 676	engineered mutation
Tyr 116	Phe 715	engineered mutation
Met 151	Gln 750	engineered mutation
Cys 258	Gln 857	engineered mutation
Ser 280	Cys 879	engineered mutation
Asn 498	Asp 1097	engineered mutation

B, D: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	257925.4
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	11
	Total formula weight	1391.9
All*	Total formula weight	260001.9

*Water molecules are not included.