5TXP
STRUCTURE OF Q151M complex (A62V, V75I, F77L, F116Y, Q151M) mutant HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DDATP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C | HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT | polymer | 556 | 64049.5 | 2 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) In PDB | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) | Pr160Gag-Pol |
2 | B, D | HIV-1 REVERSE TRANSCRIPTASE P61 SUBUNIT | polymer | 428 | 50039.5 | 2 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) | Pr160Gag-Pol |
3 | T, E | DNA (5'-D(*TP*GP*GP*TP*CP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*G)-3') | polymer | 27 | 8383.4 | 2 | Human immunodeficiency virus 1 | ||
4 | P, F | DNA (5'-D(*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*G)-3') | polymer | 21 | 6490.3 | 2 | Human immunodeficiency virus 1 | ||
5 | G, H | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900003) | sucrose | ||
6 | A, C | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
7 | A, C | 2',3'-dideoxyadenosine triphosphate | non-polymer | 475.2 | 2 | Chemie (DDS) | |||
8 | B | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
9 | B, T, D | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
10 | water | water | 18.0 | 101 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 554 (UniProt: P03366)
B, D: 1 - 428 (UniProt: P03366)
PDB | External Database | Details |
---|---|---|
Met -1 | - | initiating methionine |
Val 0 | - | expression tag |
Val 62 | Ala 661 | engineered mutation |
Ile 75 | Val 674 | engineered mutation |
Leu 77 | Phe 676 | engineered mutation |
Tyr 116 | Phe 715 | engineered mutation |
Met 151 | Gln 750 | engineered mutation |
Cys 258 | Gln 857 | engineered mutation |
Ser 280 | Cys 879 | engineered mutation |
Asn 498 | Asp 1097 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Ser 280 | Cys 879 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 257925.4 | |
Branched | Number of molecules | 2 |
Total formula weight | 684.6 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 1391.9 | |
All* | Total formula weight | 260001.9 |