5TWR
Pre-catalytic ternary complex of human Polymerase Mu (H329A) mutant with incoming nonhydrolyzable UMPNPP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed DNA/RNA polymerase mu | polymer | 354 | 39777.1 | 1 | UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (Human) | Pol Mu,Terminal transferase |
| 2 | B (T) | DNA (5'-D(*CP*GP*GP*CP*AP*TP*AP*CP*G)-3') | polymer | 9 | 2740.8 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*CP*GP*TP*A)-3') | polymer | 4 | 1190.8 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 1 | synthetic construct | ||
| 5 | E (A) | 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine | non-polymer | 483.2 | 1 | Chemie (2KH) | |||
| 6 | F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | H (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 8 | I, K, N (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 9 | J (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 10 | L, M (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 11 | O, P, Q, R (A, T, P, D) | water | water | 18.0 | 353 | Chemie (HOH) |
Sequence modifications
A: 134 - 494 (UniProt: Q9NP87)
| PDB | External Database | Details |
|---|---|---|
| Gly 129 | - | expression tag |
| Ser 130 | - | expression tag |
| Ala 131 | - | expression tag |
| Ala 132 | - | expression tag |
| Ala 133 | - | expression tag |
| Ala 329 | His 329 | engineered mutation |
| - | Pro 398 | deletion |
| - | Gly 399 | deletion |
| - | Ala 400 | deletion |
| - | Ala 401 | deletion |
| - | Val 402 | deletion |
| - | Gly 403 | deletion |
| - | Gly 404 | deletion |
| - | Ser 405 | deletion |
| - | Thr 406 | deletion |
| - | Arg 407 | deletion |
| - | Pro 408 | deletion |
| - | Cys 409 | deletion |
| Gly 410 | Pro 410 | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 44900.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1023.6 | |
| All* | Total formula weight | 45924.1 |
