5TTD

Minor pilin FctB from S. pyogenes with engineered intramolecular isopeptide bond

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Maltose-binding periplasmic protein,Pilin isopeptide linkage domain protein	polymer	499	55111.0	2	UniProt (P0AEY0) UniProt (U2UUZ4) Pfam (PF01547) Pfam (PF12892) UniProt (by SIFTS) (P0AEX9) UniProt (by SIFTS) (D2KPJ6) In PDB	Escherichia coli	MBP,MMBP,Maltodextrin-binding protein
2	C, D	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	2	In PDB BIRD (PRD_900009) GlyTouCan (G96370VA)		alpha-maltotriose
3	A, B	FORMIC ACID	non-polymer		46.0	4	Chemie (FMT)
4		water	water		18.0	277	Chemie (HOH)

Sequence modifications

A, B: 13 - 372 (UniProt: P0AEY0)

PDB	External Database	Details
Met 1	-	initiating methionine
Ser 2	-	expression tag
Tyr 3	-	expression tag
Tyr 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Met 12	-	expression tag
Gln 28	Glu 48	conflict
Asn 373	-	linker
Ala 374	-	linker
Gly 375	-	linker
Gly 376	-	linker

A, B: 377 - 499 (UniProt: U2UUZ4)

PDB	External Database	Details
Lys 390	Asn 39	conflict
Phe 403	Ile 52	conflict
Glu 444	Gln 93	conflict
Asn 494	Pro 143	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	110222.0
Branched	Number of molecules	2
	Total formula weight	1008.9
Non-Polymers*	Number of molecules	4
	Total formula weight	184.1
All*	Total formula weight	111415.0

*Water molecules are not included.