5TQL

Crystal structure of TIM-Barrel protein HisF-C9S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Imidazole glycerol phosphate synthase subunit HisF	polymer	275	30043.2	2	UniProt (Q9X0C6) Pfam (PF00977)	Thermotoga maritima	IGP synthase cyclase subunit,IGP synthase subunit HisF,ImGP synthase subunit HisF,IGPS subunit HisF
2	C, D, E, F (A, B)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
3	G, H (A, B)	water	water		18.0	413	Chemie (HOH)

Sequence modifications

A, B: 2 - 253 (UniProt: Q9X0C6)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 9	Cys 9	engineered mutation
Ser 21	Thr 21	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60086.5
Non-Polymers*	Number of molecules	4
	Total formula weight	379.9
All*	Total formula weight	60466.4

*Water molecules are not included.