5TPQ

E. coli alkaline phosphatase D101A, D153A, R166S, E322A, K328A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Alkaline phosphatase	polymer	446	46560.7	2	UniProt (P00634) Pfam (PF00245) In PDB	Escherichia coli (strain K12)	APase
2	A, B	ZINC ION	non-polymer		65.4	7	Chemie (ZN)
3	A, B	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4		water	water		18.0	411	Chemie (HOH)

Sequence modifications

A, B: 8 - 449 (UniProt: P00634)

PDB	External Database	Details
Ala 101	Asp 123	engineered mutation
Ala 153	Asp 175	engineered mutation
Ser 166	Arg 188	engineered mutation
Ala 322	Glu 344	engineered mutation
Ala 328	Lys 350	engineered mutation
Trp 450	-	expression tag
Ser 451	-	expression tag
His 452	-	expression tag
Pro 453	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93121.5
Non-Polymers*	Number of molecules	9
	Total formula weight	647.8
All*	Total formula weight	93769.3

*Water molecules are not included.