5TPQ
E. coli alkaline phosphatase D101A, D153A, R166S, E322A, K328A mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alkaline phosphatase | polymer | 446 | 46560.7 | 2 | UniProt (P00634) Pfam (PF00245) | Escherichia coli (strain K12) | APase |
| 2 | C, D, E, G, H... (A, B) | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) | |||
| 3 | F, K (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 4 | L, M (A, B) | water | water | 18.0 | 411 | Chemie (HOH) |
Sequence modifications
A, B: 8 - 449 (UniProt: P00634)
| PDB | External Database | Details |
|---|---|---|
| Ala 101 | Asp 123 | engineered mutation |
| Ala 153 | Asp 175 | engineered mutation |
| Ser 166 | Arg 188 | engineered mutation |
| Ala 322 | Glu 344 | engineered mutation |
| Ala 328 | Lys 350 | engineered mutation |
| Trp 450 | - | expression tag |
| Ser 451 | - | expression tag |
| His 452 | - | expression tag |
| Pro 453 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 93121.5 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 647.8 | |
| All* | Total formula weight | 93769.3 |
