5TPQ
E. coli alkaline phosphatase D101A, D153A, R166S, E322A, K328A mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Alkaline phosphatase | polymer | 446 | 46560.7 | 2 | UniProt (P00634) Pfam (PF00245) In PDB | Escherichia coli (strain K12) | APase |
2 | A, B | ZINC ION | non-polymer | 65.4 | 7 | Chemie (ZN) | |||
3 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
4 | water | water | 18.0 | 411 | Chemie (HOH) |
Sequence modifications
A, B: 8 - 449 (UniProt: P00634)
PDB | External Database | Details |
---|---|---|
Ala 101 | Asp 123 | engineered mutation |
Ala 153 | Asp 175 | engineered mutation |
Ser 166 | Arg 188 | engineered mutation |
Ala 322 | Glu 344 | engineered mutation |
Ala 328 | Lys 350 | engineered mutation |
Trp 450 | - | expression tag |
Ser 451 | - | expression tag |
His 452 | - | expression tag |
Pro 453 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 93121.5 | |
Non-Polymers* | Number of molecules | 9 |
Total formula weight | 647.8 | |
All* | Total formula weight | 93769.3 |