5TMF
Re-refinement of thermus thermophilus RNA polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 315 | 35056.2 | 2 | UniProt (Q9Z9H6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (Q5SHR6) | Thermus thermophilus | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1119 | 125436.5 | 1 | UniProt (Q8RQE9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Thermus thermophilus | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1524 | 170997.4 | 1 | UniProt (Q8RQE8) Pfam (PF04997) Pfam (PF21668) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Thermus thermophilus | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 99 | 11521.2 | 1 | UniProt (Q72ID6) Pfam (PF01192) UniProt (by SIFTS) (Q8RQE7) | Thermus thermophilus | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor SigA | polymer | 423 | 48598.0 | 1 | UniProt (Q72L95) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (Q5SKW1) | Thermus thermophilus | |
| 6 | G, K (A, D) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 7 | H (C) | methyl [(1E,5R)-5-{(3S)-3-[(2E,4E)-2,5-dimethylocta-2,4-dienoyl]-2,4-dioxo-3,4-dihydro-2H-pyran-6-yl}hexylidene]carbamate | non-polymer | 417.5 | 1 | Chemie (NE6) | |||
| 8 | I, J (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 9 | L, M (D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 10 | N, O, P, Q, R (A, B, C, D, F) | water | water | 18.0 | 49 | Chemie (HOH) |
Sequence modifications
E: 1 - 99 (UniProt: Q72ID6)
F: 1 - 423 (UniProt: Q72L95)
| PDB | External Database | Details |
|---|---|---|
| Glu 61 | Val 61 | conflict |
| Ile 92 | Leu 92 | conflict |
| Gly 95 | Val 95 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Thr 46 | Ala 46 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 426665.5 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 786.9 | |
| All* | Total formula weight | 427452.4 |
