5TIN

Crystal Structure of Human Glycine Receptor alpha-3 Mutant N38Q Bound to AM-3607

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Glycine receptor subunit alpha-3	polymer	362	41871.4	5	UniProt (O75311) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (Human)
2	F, I, L, O, R (A, B, C, D, E)	(3S,3aS,9bS)-2-[(2H-1,3-benzodioxol-5-yl)sulfonyl]-3,5-dimethyl-1,2,3,3a,5,9b-hexahydro-4H-pyrrolo[3,4-c][1,6]naphthyridin-4-one	non-polymer		401.4	5	Chemie (7C6)
3	G, J, N, P, S (A, B, C, D, E)	GLYCINE	non-polymer		75.1	5	Chemie (GLY)
4	H, K, M, Q, U (A, B, C, D, E)	ZINC ION	non-polymer		65.4	5	Chemie (ZN)
5	T (E)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	V, W, X, Y, Z (A, B, C, D, E)	water	water		18.0	400	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 309 (UniProt: O75311)

PDB	External Database	Details
Gln 38	Asn 71	engineered mutation

A, B, C, D, E: 313 - 354 (UniProt: O75311)

PDB	External Database	Details
Ala 310	-	linker
Gly 311	-	linker
Thr 312	-	linker
Trp 355	-	expression tag
Ser 356	-	expression tag
His 357	-	expression tag
Pro 358	-	expression tag
Gln 359	-	expression tag
Phe 360	-	expression tag
Glu 361	-	expression tag
Lys 362	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	209357.1
Non-Polymers*	Number of molecules	16
	Total formula weight	2745.0
All*	Total formula weight	212102.1

*Water molecules are not included.