5TIF
x-ray structure of acyl-CoA thioesterase I, TesA, triple mutant M141L/Y145K/L146K in complex with octanoic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Acyl-CoA thioesterase I | polymer | 185 | 20685.5 | 1 | UniProt (P0ADA1) Pfam (PF00657) | Escherichia coli | Lecithinase B,Lysophospholipase L1,Protease I |
| 2 | B (A) | OCTANOIC ACID (CAPRYLIC ACID) | non-polymer | 144.2 | 1 | Chemie (OCA) | |||
| 3 | C (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 4 | D (A) | water | water | 18.0 | 422 | Chemie (HOH) |
Sequence modifications
A: 2 - 182 (UniProt: P0ADA1)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Met 1 | - | expression tag |
| Leu 141 | Met 167 | engineered mutation |
| Lys 145 | Tyr 171 | engineered mutation |
| Lys 146 | Leu 172 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 20685.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 250.3 | |
| All* | Total formula weight | 20935.9 |
