5TDV
Intermediate O2 diiron complex in the Q228A variant of Toluene 4-moonoxygenase (T4moHD)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | Toluene-4-monooxygenase system protein A | polymer | 500 | 58099.2 | 2 | UniProt (Q00456) Pfam (PF02332) In PDB | Pseudomonas mendocina | Toluene-4-monooxygenase hydroxylase subunit,T4moH |
2 | B, F | Toluene-4-monooxygenase system protein E | polymer | 327 | 38410.2 | 2 | UniProt (Q00460) Pfam (PF02332) In PDB | Pseudomonas mendocina | T4moE |
3 | C, G | Toluene-4-monooxygenase system protein B | polymer | 84 | 9601.0 | 2 | UniProt (Q00457) Pfam (PF06234) In PDB | Pseudomonas mendocina | T4moB |
4 | E, H | Toluene-4-monooxygenase system protein D | polymer | 103 | 11642.1 | 2 | UniProt (Q00459) Pfam (PF02406) In PDB | Pseudomonas mendocina | T4mo effector protein D,T4moD,Toluene-4-monooxygenase effector protein |
5 | A, D | PEROXIDE ION | non-polymer | 32.0 | 2 | Chemie (PER) | |||
6 | A, D | FE (III) ION | non-polymer | 55.8 | 4 | Chemie (FE) | |||
7 | water | water | 18.0 | 1509 | Chemie (HOH) |
Sequence modifications
A, D: 1 - 500 (UniProt: Q00456)
B, F: 0 - 326 (UniProt: Q00460)
E, H: 0 - 102 (UniProt: Q00459)
PDB | External Database | Details |
---|---|---|
Ala 228 | Gln 228 | engineered mutation |
Trp 336 | Leu 336 | conflict |
Tyr 337 | Asp 337 | conflict |
Asp 382 | Asn 382 | conflict |
Asp 465 | Glu 465 | conflict |
PDB | External Database | Details |
---|---|---|
Tyr 239 | Trp 240 | conflict |
PDB | External Database | Details |
---|---|---|
Gln 5 | Asp 6 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 235505.1 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 287.4 | |
All* | Total formula weight | 235792.4 |