5TDL

Crystal structure of prefusion-stabilized bovine RSV fusion glycoprotein (single-chain DS2-v1 variant: strain 391-2 sc9 DS-Cav1 Q98C Q361C)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Bovine prefusion RSV F glycoprotein	polymer	531	59002.4	1	UniProt (P22167) UniProt (P10104) Pfam (PF00523) In PDB	Bovine respiratory syncytial virus (strain Copenhagen) (BRS)	Collar protein,Whisker antigen control protein
2	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A: 1 - 105 (UniProt: P22167)

PDB	External Database	Details
Cys 98	Gln 98	engineered mutation

A: 143 - 513 (UniProt: P22167)

PDB	External Database	Details
Ser 144	Ile 144	engineered mutation
Cys 155	Ser 155	engineered mutation
Phe 190	Ser 190	engineered mutation
Leu 207	Val 207	engineered mutation
Cys 290	Ser 290	engineered mutation
Cys 361	Gln 361	engineered mutation
Ser 514	-	linker
Ala 515	-	linker
Ile 516	-	linker

A: 517 - 544 (UniProt: P10104)

PDB	External Database	Details
Gly 517	Ala 457	linker
Leu 539	Phe 479	variant
Gly 545	-	expression tag
Gly 546	-	expression tag
Leu 547	-	expression tag
Val 548	-	expression tag
Pro 549	-	expression tag
Arg 550	-	expression tag
Gly 551	-	expression tag
Ser 552	-	expression tag
His 553	-	expression tag
His 554	-	expression tag
His 555	-	expression tag
His 556	-	expression tag
His 557	-	expression tag
His 558	-	expression tag
Ser 559	-	expression tag
Ala 560	-	expression tag
Trp 561	-	expression tag
Ser 562	-	expression tag
His 563	-	expression tag
Pro 564	-	expression tag
Gln 565	-	expression tag
Phe 566	-	expression tag
Glu 567	-	expression tag
Lys 568	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	59002.4
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	59223.6

*Water molecules are not included.