5TD4
Starch binding sites on the Human pancreatic alpha amylase D300N variant complexed with an octaose substrate.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pancreatic alpha-amylase | polymer | 496 | 55930.3 | 1 | UniProt (P04746) Pfam (PF00128) Pfam (PF02806) | Homo sapiens (Human) | PA,1,4-alpha-D-glucan glucanohydrolase |
| 2 | B, C (B, C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 2 | In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ) | alpha-maltotetraose | ||
| 3 | D, E (D, E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 990.9 | 2 | In PDB BIRD (PRD_900035) GlyTouCan (G91368RN) | alpha-maltohexaose | ||
| 4 | F (F) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 5 | G (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | H (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 7 | I (A) | water | water | 18.0 | 157 | Chemie (HOH) |
Sequence modifications
A: 1 - 496 (UniProt: P04746)
| PDB | External Database | Details |
|---|---|---|
| Asn 300 | Asp 315 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 55930.3 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 3657.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 75.5 | |
| All* | Total formula weight | 59663.0 |
