5T5W

Structure of an affinity matured lambda-IFN/IFN-lambdaR1/IL-10Rbeta receptor complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Interleukin-10 receptor subunit beta	polymer	214	25045.9	1	UniProt (Q08334) Pfam (PF01108) Pfam (PF09294)	Homo sapiens (Human)	IL-10RB,Cytokine receptor class-II member 4,Cytokine receptor family 2 member 4,CRF2-4,Interleukin-10 receptor subunit 2,IL-10R2
2	B (B)	Interferon lambda receptor 1	polymer	218	24703.3	1	UniProt (Q8IU57) Pfam (PF01108)	Homo sapiens (Human)	IFN-lambda-R1,Cytokine receptor class-II member 12,Cytokine receptor family 2 member 12,CRF2-12,Interleukin-28 receptor subunit alpha,IL-28RA,Likely interleukin or cytokine receptor 2,LICR2
3	C (C)	Interferon lambda-3	polymer	176	19911.9	1	UniProt (Q8IZI9) Pfam (PF15177)	Homo sapiens (Human)	IFN-lambda-3,Cytokine Zcyto22,Interleukin-28B,IL-28B,Interleukin-28C,IL-28C
4	D, E, F (B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
5	G, H, I (A, B, C)	water	water		18.0	18	Chemie (HOH)

Sequence modifications

A: 20 - 220 (UniProt: Q08334)

PDB	External Database	Details
Gly 18	-	expression tag
Ser 19	-	engineered mutation
Gln 49	Asn 49	engineered mutation
Gln 68	Asn 68	engineered mutation
Gln 102	Asn 102	engineered mutation
Gln 161	Asn 161	engineered mutation
Ala 221	-	expression tag
Ala 222	-	expression tag
Ala 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag

B: 1 - 206 (UniProt: Q8IU57)

PDB	External Database	Details
Thr -2	-	expression tag
Ser -1	-	expression tag
Arg 0	-	expression tag
Ala 207	-	expression tag
Ala 208	-	expression tag
Ala 209	-	expression tag
Leu 210	-	expression tag
Glu 211	-	expression tag
Val 212	-	expression tag
Leu 213	-	expression tag
Phe 214	-	expression tag
Gln 215	-	expression tag

C: 1 - 162 (UniProt: Q8IZI9)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Arg 15	Gln 48	engineered mutation
Asp 73	Glu 106	engineered mutation
Arg 120	His 153	engineered mutation
Ala 150	Thr 183	engineered mutation
Glu 163	-	expression tag
Ala 164	-	expression tag
Ala 165	-	expression tag
Ala 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	69661.2
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	70324.8

*Water molecules are not included.