5T4G

Crystal structure of BhGH81 in complex with laminarin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glycoside Hydrolase	polymer	783	88442.0	1	UniProt (Q9KG76) Pfam (PF03639) Pfam (PF17652) In PDB	Bacillus halodurans
2	B	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose	branched		504.4	1	In PDB GlyTouCan (G51422BS)
3	C	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900024) GlyTouCan (G36535HU)		beta-laminaribiose
4	D	beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose-(1-3)-beta-D-glucopyranose	branched		1639.4	1	In PDB GlyTouCan (G35426BM)
5	A	PHOSPHATE ION	non-polymer		95.0	12	Chemie (PO4)
6	A	1,2-ETHANEDIOL	non-polymer		62.1	17	Chemie (EDO)
7		water	water		18.0	657	Chemie (HOH)

Sequence modifications

A: 28 - 789 (UniProt: Q9KG76)

PDB	External Database	Details
Met 7	-	initiating methionine
Gly 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
Gly 19	-	expression tag
Leu 20	-	expression tag
Val 21	-	expression tag
Pro 22	-	expression tag
Arg 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	88442.0
Branched	Number of molecules	3
	Total formula weight	2486.2
Non-Polymers*	Number of molecules	29
	Total formula weight	2194.8
All*	Total formula weight	93123.0

*Water molecules are not included.