5T47

Crystal structure of the D. melanogaster eIF4E-eIF4G complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Eukaryotic translation initiation factor 4E	polymer	184	21249.0	2	UniProt (P48598) Pfam (PF01652)	Drosophila melanogaster (Fruit fly)	eIF4E,eIF-4F 25 kDa subunit,mRNA cap-binding protein
2	B, D (B, D)	Eukaryotic translation initiation factor 4G, isoform A	polymer	64	7410.4	2	UniProt (O61380) Pfam (PF21140)	Drosophila melanogaster (Fruit fly)	Eukaryotic translation initiation factor 4G,isoform C,FI02056p,Translation initiation factor eIF4G
3	E, F (C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	G, H, I, J (A, B, C, D)	water	water		18.0	157	Chemie (HOH)

Sequence modifications

A, C: 69 - 248 (UniProt: P48598)

PDB	External Database	Details
Gly 65	-	expression tag
Pro 66	-	expression tag
His 67	-	expression tag
Met 68	-	expression tag

B, D: 601 - 660 (UniProt: O61380)

PDB	External Database	Details
Gly 597	-	expression tag
Pro 598	-	expression tag
His 599	-	expression tag
Met 600	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	57318.9
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	57503.0

*Water molecules are not included.