5SYO

Crystal structure of a chimeric acetylcholine binding protein from Aplysia californica (Ac-AChBP) containing loop C from the human alpha 3 nicotinic acetylcholine receptor in complex with Cytisine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E	Soluble acetylcholine receptor, Neuronal acetylcholine receptor subunit alpha-3 chimera	polymer	230	26315.3	5	UniProt (Q8WSF8) UniProt (P32297) Pfam (PF02931) In PDB	Aplysia californica Homo sapiens (California sea hare)
2	C, E	(1R,5S)-1,2,3,4,5,6-HEXAHYDRO-8H-1,5-METHANOPYRIDO[1,2-A][1,5]DIAZOCIN-8-ONE	non-polymer		190.2	2	Chemie (C5E)
3		water	water		18.0	309	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 181 (UniProt: Q8WSF8)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Leu 0	-	expression tag
Trp 55	Tyr 72	engineered mutation

A, B, C, D, E: 182 - 197 (UniProt: P32297)
A, B, C, D, E: 198 - 219 (UniProt: Q8WSF8)

PDB	External Database	Details
Val 199	Asn 216	engineered mutation
Ser 220	-	expression tag
Arg 221	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	131576.4
Non-Polymers*	Number of molecules	2
	Total formula weight	380.5
All*	Total formula weight	131956.9

*Water molecules are not included.