5SWM
BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Ribonuclease H | polymer | 142 | 16329.5 | 2 | UniProt (Q9KEI9) In PDB | Bacillus halodurans | RNase H |
2 | C | RNA (12-MER) | polymer | 12 | 3796.2 | 1 | synthetic construct | ||
3 | D | DNA (12-MER) | polymer | 12 | 3767.3 | 1 | synthetic construct | ||
4 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
6 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
7 | D | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
8 | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A, B: 59 - 196 (UniProt: Q9KEI9)
PDB | External Database | Details |
---|---|---|
Gly 55 | - | expression tag |
Ser 56 | - | expression tag |
His 57 | - | expression tag |
Met 58 | - | expression tag |
Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 40222.4 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 256.7 | |
All* | Total formula weight | 40479.1 |