5SWM
BACILLUS HALODURANS RNASE H MUTANT D132N IN COMPLEX WITH 12-MER FRNA/DNA HYBRID
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Ribonuclease H | polymer | 142 | 16329.5 | 2 | UniProt (Q9KEI9) | Bacillus halodurans | RNase H |
| 2 | C (C) | RNA (12-MER) | polymer | 12 | 3796.2 | 1 | synthetic construct | ||
| 3 | D (D) | DNA (12-MER) | polymer | 12 | 3767.3 | 1 | synthetic construct | ||
| 4 | E (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 5 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | G (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 7 | H (D) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 8 | I, J, K, L (A, B, C, D) | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A, B: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | expression tag |
| Ser 56 | - | expression tag |
| His 57 | - | expression tag |
| Met 58 | - | expression tag |
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 40222.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 256.7 | |
| All* | Total formula weight | 40479.1 |
