5SUP

Crystal structure of the Sub2-Yra1 complex in association with RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	ATP-dependent RNA helicase SUB2	polymer	390	44462.9	3	UniProt (Q07478) Pfam (PF00270) Pfam (PF00271)	Saccharomyces cerevisiae (Baker's yeast)	Suppressor of BRR1 protein 2
2	D, E, F (G, H, I)	RNA annealing protein YRA1	polymer	32	3679.2	3	UniProt (Q12159)	Saccharomyces cerevisiae (Baker's yeast)
3	G, H, I (D, E, F)	RNA (5'-R(P*UP*UP*UP*UP*UP*U)-3')	polymer	15	4547.5	3		SYNTHETIC CONSTRUCT
4	J, M, P (A, B, C)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	3	Chemie (ADP)
5	K, N, Q (A, B, C)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	3	Chemie (BEF)
6	L, O, R (A, B, C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
7	S, T, U (A, B, C)	water	water		18.0	27	Chemie (HOH)

Sequence modifications

A, B, C: 61 - 446 (UniProt: Q07478)

PDB	External Database	Details
Gly 57	-	expression tag
Ala 58	-	expression tag
Met 59	-	expression tag
Gly 60	-	expression tag

G, H, I: 200 - 226 (UniProt: Q12159)

PDB	External Database	Details
Gly 195	-	expression tag
Ala 196	-	expression tag
Met 197	-	expression tag
Gly 198	-	expression tag
Ser 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	158068.9
Non-Polymers*	Number of molecules	9
	Total formula weight	1552.5
All*	Total formula weight	159621.5

*Water molecules are not included.