5REQ

Methylmalonyl-COA MUTASE, Y89F Mutant, substrate complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	PROTEIN (METHYLMALONYL-COA MUTASE ALPHA-SUBUNIT)	polymer	727	80121.9	2	UniProt (P11653) Pfam (PF01642) Pfam (PF02310)	Propionibacterium freudenreichii subsp. shermanii
2	B, D (B, D)	PROTEIN (METHYLMALONYL-COA MUTASE BETA-SUBUNIT)	polymer	637	69430.2	2	UniProt (P11652) Pfam (PF01642)	Propionibacterium freudenreichii subsp. shermanii
3	E, J (A, C)	SUCCINYL(CARBADETHIA)-COENZYME A	non-polymer		849.6	2	Chemie (SCD)
4	F, K (A, C)	METHYLMALONYL(CARBADETHIA)-COENZYME A	non-polymer		849.6	2	Chemie (MCD)
5	G, L (A, C)	COBALAMIN	non-polymer		1330.4	2	Chemie (B12)
6	H, I, M, N (B, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
7	O, P, Q, R (A, B, C, D)	water	water		18.0	1094	Chemie (HOH)

Sequence modifications

A, C: 2 - 728 (UniProt: P11653)

PDB	External Database	Details
Phe 89	Tyr 89	engineered mutation

B, D: 2 - 638 (UniProt: P11652)

PDB	External Database	Details
Gly 203	Ala 203	SEE REMARK 999
Glu 330	Asp 330	SEE REMARK 999
Leu 331	Val 331	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	299104.1
Non-Polymers*	Number of molecules	10
	Total formula weight	6427.4
All*	Total formula weight	305531.4

*Water molecules are not included.