5OWF

Structure of a LAO-binding protein mutant with glutamine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lysine/arginine/ornithine-binding periplasmic protein	polymer	259	28376.9	1	UniProt (P02911) Pfam (PF00497)	Salmonella typhimurium	LAO-binding protein
2	B, C, D, E, F... (A)	1,2-ETHANEDIOL	non-polymer		62.1	18	Chemie (EDO)
3	T (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4	U (A)	GLUTAMINE	non-polymer		146.1	1	Chemie (GLN)
5	V, W (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6	X (A)	water	water		18.0	145	Chemie (HOH)

Sequence modifications

A: 22 - 259 (UniProt: P02911)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag
Lys 138	Leu 139	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28376.9
Non-Polymers*	Number of molecules	22
	Total formula weight	1553.7
All*	Total formula weight	29930.6

*Water molecules are not included.