5OQO

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (crystal form II)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Condensin complex subunit 3	polymer	871	99715.9	1	UniProt (Q06680) Pfam (PF12719) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	CAPG homolog
2	B	Condensin complex subunit 2	polymer	152	18134.6	1	UniProt (P38170) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Barren homolog,CAPH homolog
3	C, D	DNA (5'-D(*GP*AP*TP*GP*TP*GP*TP*AP*GP*CP*TP*AP*CP*AP*CP*AP*TP*C)-3')	polymer	18	5515.6	2		synthetic construct
4	A	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
5	A	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)

Sequence modifications

A: 6 - 932 (UniProt: Q06680)

PDB	External Database	Details
Met 5	-	initiating methionine
-	Gln 499	deletion
-	Glu 500	deletion
-	Glu 501	deletion
-	Lys 502	deletion
-	Ile 503	deletion
-	Lys 504	deletion
-	Ser 505	deletion
-	Lys 506	deletion
-	Lys 507	deletion
-	Ile 508	deletion
-	Asn 509	deletion
-	Arg 510	deletion
-	Arg 511	deletion
-	Asn 512	deletion
-	Glu 513	deletion
-	Thr 514	deletion
-	Ser 515	deletion
-	Val 516	deletion
-	Asp 517	deletion
-	Glu 518	deletion
-	Glu 519	deletion
-	Asp 520	deletion
-	Glu 521	deletion
-	Asn 522	deletion
-	Gly 523	deletion
-	Thr 524	deletion
-	His 525	deletion
-	Asn 526	deletion
-	Asp 527	deletion
-	Glu 528	deletion
-	Val 529	deletion
-	Asn 530	deletion
-	Glu 531	deletion
-	Asp 532	deletion
-	Glu 533	deletion
-	Glu 534	deletion
-	Asp 535	deletion
-	Asp 536	deletion
-	Asn 537	deletion
-	Ile 538	deletion
-	Ser 539	deletion
-	Ser 540	deletion
-	Phe 541	deletion
-	His 542	deletion
-	Ser 543	deletion
-	Ala 544	deletion
-	Val 545	deletion
-	Glu 546	deletion
-	Asn 547	deletion
-	Leu 548	deletion
-	Val 549	deletion
-	Gln 550	deletion
-	Gly 551	deletion
-	Asn 552	deletion
-	Gly 553	deletion
-	Asn 554	deletion
-	Val 555	deletion

B: 384 - 529 (UniProt: P38170)

PDB	External Database	Details
Gly 378	-	expression tag
Pro 379	-	expression tag
Leu 380	-	expression tag
Gly 381	-	expression tag
His 382	-	expression tag
Met 383	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128881.7
Non-Polymers*	Number of molecules	4
	Total formula weight	544.6
All*	Total formula weight	129426.3

*Water molecules are not included.