5OQH

Crystal Structure of a disulfide trapped single chain trimer composed of the MHC I heavy chain H-2Kb Y84C K66A mutant, beta-2microglobulin, and ovalbumin-derived peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-2-microglobulin,H-2 class I histocompatibility antigen, K-B alpha chain	polymer	431	48123.5	2	UniProt (P01887) UniProt (P01901) Pfam (PF07654) Pfam (PF00129) In PDB	Mus musculus (Mouse)	H-2K(B)
2		water	water		18.0	462	Chemie (HOH)

Sequence modifications

A, B: 24 - 112 (UniProt: P01887)

PDB	External Database	Details
Ser 1	-	insertion
Ile 2	-	insertion
Ile 3	-	insertion
Asn 4	-	insertion
Phe 5	-	insertion
Glu 6	-	insertion
Lys 7	-	insertion
Leu 8	-	insertion
Gly 9	-	expression tag
Cys 10	-	expression tag
Gly 11	-	expression tag
Ala 12	-	expression tag
Ser 13	-	expression tag
Gly	-	expression tag
Gly	-	expression tag
Gly	-	expression tag
Gly	-	expression tag
Ser	-	expression tag
Gly	-	expression tag
Gly	-	expression tag
Gly	-	expression tag
Gly	-	expression tag
Ser	-	expression tag
Pro 113	-	linker
Lys 114	-	linker
Thr 115	-	linker
Val 116	-	linker
Tyr 117	-	linker
Trp 118	-	linker
Asp 119	-	linker
Arg 120	-	linker
Asp 121	-	linker
Met 122	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Ser	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Ser	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Ser	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Gly	-	linker
Ser	-	linker

A, B: 143 - 425 (UniProt: P01901)

PDB	External Database	Details
Ala 208	Lys 87	engineered mutation
Cys 226	Tyr 105	engineered mutation
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	96247.0
All*	Total formula weight	96247.0

*Water molecules are not included.