5OPL

Crystal structure of K25E cN-II mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cytosolic purine 5'-nucleotidase	polymer	555	64088.9	1	UniProt (P49902) Pfam (PF05761)	Homo sapiens (Human)	Cytosolic 5'-nucleotidase II
2	B, C, D, E, F... (A)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
3	K (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	L (A)	water	water		18.0	364	Chemie (HOH)

Sequence modifications

A: 1 - 536 (UniProt: P49902)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Glu 25	Lys 25	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	64088.9
Non-Polymers*	Number of molecules	10
	Total formula weight	853.2
All*	Total formula weight	64942.0

*Water molecules are not included.