5ONW

X-Ray crystal structure of a nucleosome core particle with its DNA site-specifically crosslinked to the histone octamer and the two H2A/H2B dimers crosslinked via H2A N38C

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (I)	DNA (147-MER)	polymer	147	45430.1	1		Homo sapiens (Human)
2	B (J)	DNA (147-MER)	polymer	147	45421.1	1		Homo sapiens (Human)
3	C, G (A, E)	Histone H3.2	polymer	135	15217.8	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
4	D, H (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
5	E, I (C, G)	Histone H2A	polymer	129	13967.3	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (African clawed frog)
6	F, J (D, H)	Histone H2B 1.1	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
7	AA, BA, EA, FA, K... (J, D, E, I)	MANGANESE (II) ION	non-polymer		54.9	20	Chemie (MN)
8	CA, DA, GA, HA (A, C, E, G)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
9	IA, JA, KA, LA, MA... (I, J, A, B, C...)	water	water		18.0	62	Chemie (HOH)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Cys 40	Arg 41	engineered mutation
Ala 102	Gly 103	conflict
Ala 110	Cys 111	engineered mutation

C, G: 1 - 129 (UniProt: Q6AZJ8)

PDB	External Database	Details
Cys 38	Asn 39	engineered mutation

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	conflict

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	199059.8
Non-Polymers*	Number of molecules	24
	Total formula weight	1240.6
All*	Total formula weight	200300.4

*Water molecules are not included.