5ONG
X-Ray crystal structure of a nucleosome core particle with its DNA site-specifically crosslinked to the histone octamer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (I) | DNA (147-MER) | polymer | 147 | 45430.1 | 1 | Homo sapiens (Human) | ||
| 2 | B (J) | DNA (147-MER) | polymer | 147 | 45421.1 | 1 | Homo sapiens (Human) | ||
| 3 | C, G (A, E) | Histone H3.2 | polymer | 135 | 15217.8 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (B, F) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 5 | E, I (C, G) | Histone H2A | polymer | 129 | 13978.2 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) | Xenopus laevis (African clawed frog) | |
| 6 | F, J (D, H) | Histone H2B 1.1 | polymer | 122 | 13524.8 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 7 | AA, BA, CA, FA, K... (J, E, I) | MANGANESE (II) ION | non-polymer | 54.9 | 20 | Chemie (MN) | |||
| 8 | DA, EA, GA, HA (A, D, E, G) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 9 | IA, JA, KA, LA, MA... (I, J, A, B, C...) | water | water | 18.0 | 87 | Chemie (HOH) |
Sequence modifications
A, E: 1 - 135 (UniProt: P84233)
D, H: 1 - 122 (UniProt: P02281)
| PDB | External Database | Details |
|---|---|---|
| Cys 40 | Arg 41 | engineered mutation |
| Ala 102 | Gly 103 | conflict |
| Ala 110 | Cys 111 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Thr 29 | Ser 33 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 199081.7 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 1240.6 | |
| All* | Total formula weight | 200322.3 |
