5ON2

Quaternary complex of mutant T252A of E. coli leucyl-tRNA synthetase with tRNA(leu), leucyl-adenylate analogue, and post-transfer editing analogue of norvaline in the aminoacylation conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Leucine--tRNA ligase	polymer	880	99486.0	2	UniProt (P07813) Pfam (PF09334) Pfam (PF13603) Pfam (PF00133) Pfam (PF08264) In PDB	Escherichia coli (strain K12)	Leucyl-tRNA synthetase,LeuRS
2	B, E	tRNA(leu)	polymer	87	28052.7	2	GenBank (1231762938)	Escherichia coli
3	A, D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A, D	5'-O-(L-leucylsulfamoyl)adenosine	non-polymer		459.5	2	Chemie (LSS)
5	A, D	2'-(L-NORVALYL)AMINO-2'-DEOXYADENOSINE	non-polymer		365.4	2	Chemie (VRT)
6	B, E	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, D: 1 - 860 (UniProt: P07813)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 252	Thr 252	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	255077.3
Non-Polymers*	Number of molecules	8
	Total formula weight	1829.2
All*	Total formula weight	256906.4

*Water molecules are not included.