5OMQ
Ternary complex of 9N DNA polymerase in the replicative state with three metal ions in the active site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 775 | 89826.6 | 1 | UniProt (Q56366) Pfam (PF03104) Pfam (PF00136) | Thermococcus sp. (strain 9oN-7) | |
| 2 | B (T) | DNA Template | polymer | 16 | 4930.2 | 1 | synthetic construct | ||
| 3 | C (P) | DNA Primer | polymer | 12 | 3617.4 | 1 | synthetic construct | ||
| 4 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | E (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 6 | F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | H (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 8 | I (A) | 2'-DEOXYADENOSINE 5'-TRIPHOSPHATE | non-polymer | 491.2 | 1 | Chemie (DTP) | |||
| 9 | J, K, L (A, T, P) | water | water | 18.0 | 90 | Chemie (HOH) |
Sequence modifications
A: 1 - 775 (UniProt: Q56366)
| PDB | External Database | Details |
|---|---|---|
| Ala 141 | Asp 141 | engineered mutation |
| Ala 143 | Glu 143 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 98374.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 837.0 | |
| All* | Total formula weight | 99211.1 |
