5OLU

The crystal structure of a highly thermostable carboxyl esterase from Bacillus coagulans in complex with glycerol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha/beta hydrolase family protein	polymer	346	39171.9	1	UniProt (A0A0B5WSQ6) In PDB	Bacillus coagulans DSM 1 = ATCC 7050
2	A	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
3	A	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	A	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
6		water	water		18.0	192	Chemie (HOH)

Sequence modifications

A: 1 - 310 (UniProt: A0A0B5WSQ6)

PDB	External Database	Details
Met -35	-	initiating methionine
Arg -34	-	expression tag
Gly -33	-	expression tag
Ser -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
Gly -25	-	expression tag
Met -24	-	expression tag
Ala -23	-	expression tag
Ser -22	-	expression tag
Met -21	-	expression tag
Thr -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Gln -17	-	expression tag
Gln -16	-	expression tag
Met -15	-	expression tag
Gly -14	-	expression tag
Arg -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Tyr -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
His -3	-	expression tag
Pro -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39171.9
Non-Polymers*	Number of molecules	13
	Total formula weight	725.1
All*	Total formula weight	39897.0

*Water molecules are not included.