5OL7

Crystal structure of an inactivated Npu SICLOPPS intein with CFAHPQ extein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA-directed DNA polymerase,DNA-directed DNA polymerase	polymer	150	17406.7	1	UniProt (B2J821) UniProt (B2J066)	Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
2	B (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	C (A)	COBALT (II) ION	non-polymer		58.9	1	Chemie (CO)
4	D (A)	water	water		18.0	142	Chemie (HOH)

Sequence modifications

A: 1 - 38 (UniProt: B2J821)

PDB	External Database	Details
Val 2	Ile 2	conflict
Ala 36	Asn 36	conflict
Ala 39	-	linker
His 40	-	linker
Pro 41	-	linker
Gln 42	-	linker
Ala 43	-	linker

A: 44 - 144 (UniProt: B2J066)

PDB	External Database	Details
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	17406.7
Non-Polymers*	Number of molecules	2
	Total formula weight	99.0
All*	Total formula weight	17505.7

*Water molecules are not included.