5OL1

Crystal structure of an inactivated Ssp SICLOPPS intein with a CAFHPQ extein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase III subunit alpha,DNA polymerase III subunit alpha	polymer	162	18451.7	1	UniProt (P74750) In PDB	Synechocystis sp. (strain PCC 6803 / Kazusa)
2	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
3	A	BETA-MERCAPTOETHANOL	non-polymer		78.1	1	Chemie (BME)
4	A	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
5	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6		water	water		18.0	117	Chemie (HOH)

Sequence modifications

A: 1 - 34 (UniProt: P74750)
A: 44 - 156 (UniProt: P74750)

PDB	External Database	Details
His 35	-	linker
Ala 36	-	linker
Cso 37	-	linker
Ala 38	-	linker
Phe 39	-	linker
His 40	-	linker
Pro 41	-	linker
Gln 42	-	linker
Ala 43	-	linker
-	Lys 875	deletion
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18451.7
Non-Polymers*	Number of molecules	5
	Total formula weight	482.5
All*	Total formula weight	18934.2

*Water molecules are not included.