5OKG
Non-conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with cellobiose-6-phosphate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Putative 6-phospho-beta-galactobiosidase | polymer | 485 | 56208.7 | 4 | UniProt (W8QF82) Pfam (PF00232) In PDB | Geobacillus stearothermophilus | |
2 | E, F | 6-O-phosphono-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 422.3 | 2 | In PDB BIRD (PRD_900053) GlyTouCan (G15380AW) | beta-cellobiose-6-phosphate | ||
3 | A, B | 6-O-phosphono-beta-D-glucopyranose | non-polymer | 260.1 | 2 | Chemie (BG6) | |||
4 | A, B, C, D | GLYCEROL | non-polymer | 92.1 | 12 | Chemie (GOL) | |||
5 | A, C, D | IMIDAZOLE | non-polymer | 69.1 | 3 | Chemie (IMD) | |||
6 | water | water | 18.0 | 2187 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 478 (UniProt: W8QF82)
PDB | External Database | Details |
---|---|---|
Met -6 | - | initiating methionine |
Ile -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
Gln 170 | Glu 170 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 224834.9 | |
Branched | Number of molecules | 2 |
Total formula weight | 844.6 | |
Non-Polymers* | Number of molecules | 17 |
Total formula weight | 1832.7 | |
All* | Total formula weight | 227512.1 |