5OKE

Conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with cellobiose-6-phosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative 6-phospho-beta-galactobiosidase	polymer	485	56208.7	4	UniProt (W8QF82) Pfam (PF00232) In PDB	Geobacillus stearothermophilus
2	E, F	6-O-phosphono-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		422.3	2	In PDB BIRD (PRD_900053) GlyTouCan (G15380AW)		beta-cellobiose-6-phosphate
3	A, B	6-O-phosphono-beta-D-glucopyranose	non-polymer		260.1	2	Chemie (BG6)
4	A, B, C, D	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
5	A, C, D	IMIDAZOLE	non-polymer		69.1	3	Chemie (IMD)
6		water	water		18.0	2196	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 478 (UniProt: W8QF82)

PDB	External Database	Details
Met -6	-	initiating methionine
Ile -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 170	Glu 170	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224834.9
Branched	Number of molecules	2
	Total formula weight	844.6
Non-Polymers*	Number of molecules	17
	Total formula weight	1832.7
All*	Total formula weight	227512.1

*Water molecules are not included.