5OKA

Non-conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a 6-phospho-beta-galactosidase from Geobacillus stearothermophilus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative 6-phospho-beta-galactobiosidase	polymer	485	56208.7	4	UniProt (W8QF82) Pfam (PF00232) In PDB	Geobacillus stearothermophilus
2	A, B, C, D	GLYCEROL	non-polymer		92.1	12	Chemie (GOL)
3	A, C, D	IMIDAZOLE	non-polymer		69.1	3	Chemie (IMD)
4		water	water		18.0	2315	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 478 (UniProt: W8QF82)

PDB	External Database	Details
Met -6	-	initiating methionine
Ile -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gln 170	Glu 170	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224834.9
Non-Polymers*	Number of molecules	15
	Total formula weight	1312.4
All*	Total formula weight	226147.3

*Water molecules are not included.