5OKA
Non-conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a 6-phospho-beta-galactosidase from Geobacillus stearothermophilus
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Putative 6-phospho-beta-galactobiosidase | polymer | 485 | 56208.7 | 4 | UniProt (W8QF82) Pfam (PF00232) In PDB | Geobacillus stearothermophilus | |
2 | A, B, C, D | GLYCEROL | non-polymer | 92.1 | 12 | Chemie (GOL) | |||
3 | A, C, D | IMIDAZOLE | non-polymer | 69.1 | 3 | Chemie (IMD) | |||
4 | water | water | 18.0 | 2315 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 478 (UniProt: W8QF82)
PDB | External Database | Details |
---|---|---|
Met -6 | - | initiating methionine |
Ile -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
Gln 170 | Glu 170 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 224834.9 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 1312.4 | |
All* | Total formula weight | 226147.3 |