5OK7
Conservatively refined structure of Gan1D-E170Q, a catalytic mutant of a putative 6-phospho-beta-galactosidase from Geobacillus stearothermophilus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Putative 6-phospho-beta-galactobiosidase | polymer | 485 | 56208.7 | 4 | UniProt (W8QF82) Pfam (PF00232) | Geobacillus stearothermophilus | |
| 2 | E, F, G, H, J... (A, B, C, D) | GLYCEROL | non-polymer | 92.1 | 12 | Chemie (GOL) | |||
| 3 | I, Q, S (A, C, D) | IMIDAZOLE | non-polymer | 69.1 | 3 | Chemie (IMD) | |||
| 4 | T, U, V, W (A, B, C, D) | water | water | 18.0 | 2317 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 478 (UniProt: W8QF82)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| Ile -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Gln 170 | Glu 170 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 224834.9 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1312.4 | |
| All* | Total formula weight | 226147.3 |
