5OGL
Structure of bacterial oligosaccharyltransferase PglB in complex with an acceptor peptide and an lipid-linked oligosaccharide analog
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Undecaprenyl-diphosphooligosaccharide--protein glycotransferase | polymer | 713 | 83037.3 | 1 | UniProt (B9KDD4) Pfam (PF02516) Pfam (PF21436) Pfam (PF18527) | Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) | Protein glycosylation B |
| 2 | B (B) | Substrate mimicking peptide | polymer | 8 | 853.8 | 1 | Campylobacter jejuni | ||
| 3 | C, D (A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 4 | E (A) | [(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{Z},6~{Z},10~{Z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid | non-polymer | 651.7 | 1 | Chemie (9UB) | |||
| 5 | F (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 6 | G, H (A, B) | water | water | 18.0 | 20 | Chemie (HOH) |
Sequence modifications
A: 1 - 712 (UniProt: B9KDD4)
| PDB | External Database | Details |
|---|---|---|
| Glu 2 | Lys 2 | engineered mutation |
| Ala 17 | Cys 17 | engineered mutation |
| Ala 30 | Cys 30 | engineered mutation |
| Thr 108 | Ala 108 | engineered mutation |
| Leu 350 | Cys 350 | engineered mutation |
| Gln 535 | Asn 535 | engineered mutation |
| Pro 549 | Lys 549 | engineered mutation |
| Asn 550 | Asp 550 | engineered mutation |
| Ile 553 | Phe 553 | engineered mutation |
| Pro 556 | Asn 556 | engineered mutation |
| Pro 600 | Ala 600 | engineered mutation |
| Leu 601 | Ile 601 | engineered mutation |
| Asp 602 | Ala 602 | engineered mutation |
| Lys 606 | Thr 606 | engineered mutation |
| Gln 607 | Thr 607 | engineered mutation |
| Ile 610 | Val 610 | engineered mutation |
| Thr 611 | Met 611 | engineered mutation |
| Ser 619 | Ile 619 | engineered mutation |
| Tyr 622 | Phe 622 | engineered mutation |
| Ser 624 | Ala 624 | engineered mutation |
| Ile 627 | Val 627 | engineered mutation |
| Asn 630 | Ala 630 | engineered mutation |
| Tyr 663 | Phe 663 | engineered mutation |
| Tyr 670 | Phe 670 | engineered mutation |
| Glu 713 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 83891.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 784.5 | |
| All* | Total formula weight | 84675.6 |
