5OFK
Crystal structure of CbXyn10C variant E140Q/E248Q complexed with xyloheptaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Glycoside hydrolase family 48 | polymer | 339 | 39371.3 | 1 | UniProt (B9MKT7) Pfam (PF00331) In PDB | Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320) | |
| 2 | B | beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose-(1-4)-beta-D-xylopyranose | branched | 942.8 | 1 | In PDB GlyTouCan (G58846AG) | |||
| 3 | A | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
| 4 | A | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 2 | Chemie (MPD) | |||
| 5 | A | PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) | non-polymer | 302.4 | 1 | Chemie (PIN) | |||
| 6 | water | water | 18.0 | 569 | Chemie (HOH) |
Sequence modifications
A: 1 - 336 (UniProt: B9MKT7)
| PDB | External Database | Details |
|---|---|---|
| Asp -2 | - | expression tag |
| Lys -1 | - | expression tag |
| Met 0 | - | expression tag |
| Gln 140 | Glu 177 | engineered mutation |
| Gln 248 | Glu 285 | engineered mutation |
| Leu 319 | Phe 356 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 39371.3 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 942.8 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 923.0 | |
| All* | Total formula weight | 41237.1 |
