5OC2

Crystal structure of Asp295Cys/Lys303Cys Amadoriase I mutant from Aspergillus Fumigatus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fructosyl amine:oxygen oxidoreductase	polymer	461	51298.8	2	UniProt (O42629) Pfam (PF01266) UniProt (by SIFTS) (Q4WIF5)	Neosartorya fumigata
2	C, D (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
3	E, F (A, B)	water	water		18.0	282	Chemie (HOH)

Sequence modifications

A, B: 1 - 445 (UniProt: O42629)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
Ile -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Cys 295	Asp 295	engineered mutation
Cys 303	Lys 303	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	102597.5
Non-Polymers*	Number of molecules	2
	Total formula weight	1571.1
All*	Total formula weight	104168.6

*Water molecules are not included.