5OA5

CELLOBIOHYDROLASE I (CEL7A) FROM HYPOCREA JECORINA WITH IMPROVED THERMAL STABILITY

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Exoglucanase 1	polymer	434	46262.2	2	UniProt (P62694) Pfam (PF00840) In PDB	Hypocrea jecorina	1,4-beta-cellobiohydrolase,Exocellobiohydrolase I,CBHI,Exoglucanase I
2	A, B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
3	B	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4		water	water		18.0	546	Chemie (HOH)

Sequence modifications

A, B: 2 - 434 (UniProt: P62694)

PDB	External Database	Details
Pro 8	Ser 25	engineered mutation
Ile 41	Thr 58	engineered mutation
Ser 49	Asn 66	engineered mutation
Thr 68	Ala 85	engineered mutation
Asp 89	Asn 106	engineered mutation
Thr 92	Ser 109	engineered mutation
Asn 113	Ser 130	engineered mutation
Thr 196	Ser 213	engineered mutation
Leu 227	Pro 244	engineered mutation
Lys 249	Asp 266	engineered mutation
Pro 255	Thr 272	engineered mutation
Pro 278	Ser 295	engineered mutation
Lys 295	Glu 312	engineered mutation
Pro 296	Thr 313	engineered mutation
Tyr 332	Thr 349	engineered mutation
Asp 403	Val 420	engineered mutation
Phe 411	Ser 428	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92524.5
Non-Polymers*	Number of molecules	7
	Total formula weight	1419.3
All*	Total formula weight	93943.8

*Water molecules are not included.