5O82

Crystal Structure of R67A/E173A Mutant of alpha-L-arabinofuranosidase Ara51 from Clostridium thermocellum in complex with arabinofuranose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Intracellular exo-alpha-(1->5)-L-arabinofuranosidase	polymer	501	57371.9	6	UniProt (A3DIH0) Pfam (PF06964) In PDB	Clostridium thermocellum	ABF,Intracellular arabinan exo-alpha-(1->5)-L-arabinosidase,Arabinosidase
2	A, B, C, D, E...	alpha-L-arabinofuranose	non-polymer		150.1	6	Chemie (AHR)
3		water	water		18.0	259	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 2 - 502 (UniProt: A3DIH0)

PDB	External Database	Details
Ala 67	Arg 67	engineered mutation
Ala 173	Glu 173	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	344231.6
Non-Polymers*	Number of molecules	6
	Total formula weight	900.8
All*	Total formula weight	345132.4

*Water molecules are not included.