5O6I

Structures and dynamics of mesophilic variants from the homing endonuclease I-DmoI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G (A, F, K)	Homing endonuclease I-DmoI	polymer	200	23416.3	3	UniProt (P21505)	Desulfurococcus mucosus
2	B, E, H (C, G, L)	DNA (25-MER)	polymer	25	7707.9	3		synthetic construct
3	C, F, I (D, I, N)	DNA (25-MER)	polymer	25	7654.9	3		synthetic construct
4	J, K, M, N, P... (A, F, K)	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN)
5	L, O (A, G)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	R, S, T, U, V... (A, C, D, F, G...)	water	water		18.0	283	Chemie (HOH)

Sequence modifications

A, F, K: 2 - 188 (UniProt: P21505)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Phe 52	Ile 52	conflict
Thr 92	Ala 92	conflict
Cys 101	Phe 101	conflict
Ala 189	-	expression tag
Ala 190	-	expression tag
Ala 191	-	expression tag
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	116337.4
Non-Polymers*	Number of molecules	8
	Total formula weight	400.5
All*	Total formula weight	116737.9

*Water molecules are not included.