5O6G

Structures and dynamics of mesophilic variants from the homing endonuclease I-DmoI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G	Homing endonuclease I-DmoI	polymer	200	23445.2	3	UniProt (P21505) Pfam (PF14528) In PDB	Desulfurococcus mucosus
2	B, E, H	DNA (25-MER)	polymer	25	7707.9	3		synthetic construct
3	C, F, I	DNA (25-MER)	polymer	25	7654.9	3		synthetic construct
4	A, D, G	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN)
5	G	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	52	Chemie (HOH)

Sequence modifications

A, D, G: 2 - 188 (UniProt: P21505)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Phe 52	Ile 52	conflict
Gln 95	Leu 95	conflict
Ala 189	-	expression tag
Ala 190	-	expression tag
Ala 191	-	expression tag
Leu 192	-	expression tag
Glu 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	116424.3
Non-Polymers*	Number of molecules	7
	Total formula weight	365.1
All*	Total formula weight	116789.4

*Water molecules are not included.