5O67

Crystal structure of the FapF polypeptide transporter - F103A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	FapF	polymer	334	36393.1	1	UniProt (C4IN73)	Pseudomonas sp. UK4
2	B, C (B, C)	FapF	polymer	334	36375.1	2	UniProt (C4IN73)	Pseudomonas sp. UK4
3	D, E, F, G, H (A, B, C)	LAURYL DIMETHYLAMINE-N-OXIDE	non-polymer		229.4	5	Chemie (LDA)
4	I, J, K (C)	(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE	non-polymer		306.4	3	Chemie (C8E)
5	L, M (A, C)	water	water		18.0	13	Chemie (HOH)

Sequence modifications

A: 83 - 406 (UniProt: C4IN73)

PDB	External Database	Details
Thr 73	-	expression tag
Ser 74	-	expression tag
His 75	-	expression tag
His 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
Gly 81	-	expression tag
Thr 82	-	expression tag
Ala 103	Phe 127	engineered mutation
Met 252	Leu 276	conflict
Met 273	Leu 297	conflict

B, C: 83 - 406 (UniProt: C4IN73)

PDB	External Database	Details
Thr 73	-	expression tag
Ser 74	-	expression tag
His 75	-	expression tag
His 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
Gly 81	-	expression tag
Thr 82	-	expression tag
Ala 103	Phe 127	engineered mutation
Met 273	Leu 297	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	109143.4
Non-Polymers*	Number of molecules	8
	Total formula weight	2066.3
All*	Total formula weight	111209.7

*Water molecules are not included.