5NZ5

A C145S mutant of Nesterenkonia AN1 amidase from the nitrilase superfamily

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Amidase	polymer	283	30084.7	1	UniProt (D0VWZ1) Pfam (PF00795) In PDB	Nesterenkonia sp. 10004
2		water	water		18.0	224	Chemie (HOH)

Sequence modifications

A: 1 - 263 (UniProt: D0VWZ1)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 145	Cys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30084.7
All*	Total formula weight	30084.7

*Water molecules are not included.