5NXB

Mouse galactocerebrosidase in complex with saposin A

Replaces:  5N8K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Galactocerebrosidase	polymer	654	74596.8	2	UniProt (P54818) Pfam (PF02057) Pfam (PF17387) Pfam (PF21708)	Mus musculus (Mouse)	GALCERase,Galactocerebroside beta-galactosidase,Galactosylceramidase,Galactosylceramide beta-galactosidase
2	C, D (C, D)	Prosaposin	polymer	87	9569.9	2	UniProt (Q61207) Pfam (PF05184) Pfam (PF03489)	Mus musculus (Mouse)	Sulfated glycoprotein 1,SGP-1
3	E, F, H, I (E, F, H, I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
4	G, J (G, J)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	K, M (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
6	L, N (A, B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)

Sequence modifications

A, B: 25 - 668 (UniProt: P54818)

PDB	External Database	Details
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Ile 21	-	expression tag
Glu 22	-	expression tag
Gly 23	-	expression tag
Arg 24	-	expression tag

C, D: 0 - 84 (UniProt: Q61207)

PDB	External Database	Details
Met -2	-	initiating methionine
Gly -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	168333.4
Branched	Number of molecules	6
	Total formula weight	2870.7
Non-Polymers*	Number of molecules	4
	Total formula weight	522.6
All*	Total formula weight	171726.6

*Water molecules are not included.