5NW9

Crystal structure of the complex of Tdp1 with duplex DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tyrosyl-DNA phosphodiesterase 1	polymer	485	54731.2	2	UniProt (Q9NUW8) Pfam (PF06087)	Homo sapiens (Human)	Tyr-DNA phosphodiesterase 1
2	C (C)	DNA (5'-D(P*TP*GP*CP*GP*CP*AP*GP*TP*A)-3')	polymer	9	2755.8	1		synthetic construct
3	D, E, F (A, B, C)	water	water		18.0	57	Chemie (HOH)

Sequence modifications

A, B: 149 - 608 (UniProt: Q9NUW8)

PDB	External Database	Details
Met 124	-	initiating methionine
Gly 125	-	expression tag
Ser 126	-	expression tag
Ser 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
Ser 134	-	expression tag
Ser 135	-	expression tag
Gly 136	-	expression tag
Leu 137	-	expression tag
Val 138	-	expression tag
Pro 139	-	expression tag
Arg 140	-	expression tag
Gly 141	-	expression tag
Ser 142	-	expression tag
His 143	-	expression tag
Met 144	-	expression tag
Leu 145	-	expression tag
Glu 146	-	expression tag
Asp 147	-	expression tag
Pro 148	-	expression tag
Asn 322	Asp 322	conflict
Thr 328	Met 328	conflict
Leu 548	Phe 548	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	112218.2
All*	Total formula weight	112218.2

*Water molecules are not included.