5NSS

Cryo-EM structure of RNA polymerase-sigma54 holoenzyme with promoter DNA and transcription activator PspF intermedate complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Escherichia coli K-12	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Escherichia coli K-12	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta',DNA-directed RNA polymerase subunit beta',DNA-directed RNA polymerase subunit beta'	polymer	1449	158941.1	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Escherichia coli K-12	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta',RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta',RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192)	Escherichia coli K-12	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, G, H, I, J... (F, G, J, K, L...)	Psp operon transcriptional activator	polymer	295	33187.8	6	UniProt (P37344) Pfam (PF00158)	Escherichia coli K-12	Phage shock protein F
6	L (H)	Non-template promoter DNA	polymer	63	19404.4	1		Escherichia coli K-12
7	M (M)	RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor RpoN,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor	polymer	581	62839.6	1	UniProt (A0A0J4U551) UniProt (A0A1W1PRD8) UniProt (A0A060VKE4) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (P06223)	Klebsiella pneumoniae
8	N (I)	Template promoter DNA	polymer	63	19439.4	1		Klebsiella pneumoniae

Sequence modifications

D: 1 - 55 (UniProt: P0A8T7)
D: 56 - 97 (PDB: 5NSS)
D: 99 - 1407 (UniProt: P0A8T7)
F, G, J, K, L, N: 1 - 275 (UniProt: P37344)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

M: -111 - 7 (UniProt: A0A060VKE4)
M: 8 - 49 (PDB: 5NSS)
M: 120 - 258 (UniProt: A0A0J4U551)
M: 259 - 304 (PDB: 5NSS)
M: 306 - 396 (UniProt: A0A1W1PRD8)
M: 398 - 413 (PDB: 5NSS)
M: 415 - 477 (UniProt: A0A0J4U551)

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	693938.9
All*	Total formula weight	693938.9

*Water molecules are not included.