5NSS
Cryo-EM structure of RNA polymerase-sigma54 holoenzyme with promoter DNA and transcription activator PspF intermedate complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | D | DNA-directed RNA polymerase subunit beta',DNA-directed RNA polymerase subunit beta',DNA-directed RNA polymerase subunit beta' | polymer | 1449 | 158941.1 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta',RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta',RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
4 | E | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) In PDB | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
5 | F, G, J, K, L... | Psp operon transcriptional activator | polymer | 295 | 33187.8 | 6 | UniProt (P37344) Pfam (PF00158) In PDB | Escherichia coli K-12 | Phage shock protein F |
6 | H | Non-template promoter DNA | polymer | 63 | 19404.4 | 1 | Escherichia coli K-12 | ||
7 | M | RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor RpoN,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor,RNA polymerase sigma-54 factor | polymer | 581 | 62839.6 | 1 | UniProt (A0A0J4U551) UniProt (A0A1W1PRD8) UniProt (A0A060VKE4) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (P06223) In PDB | Klebsiella pneumoniae | |
8 | I | Template promoter DNA | polymer | 63 | 19439.4 | 1 | Klebsiella pneumoniae |
Sequence modifications
D: 1 - 55 (UniProt: P0A8T7)
D: 56 - 97 (PDB: 5NSS)
D: 99 - 1407 (UniProt: P0A8T7)
F, G, J, K, L, N: 1 - 275 (UniProt: P37344)
M: -111 - 7 (UniProt: A0A060VKE4)
M: 8 - 49 (PDB: 5NSS)
M: 120 - 258 (UniProt: A0A0J4U551)
M: 259 - 304 (PDB: 5NSS)
M: 306 - 396 (UniProt: A0A1W1PRD8)
M: 398 - 413 (PDB: 5NSS)
M: 415 - 477 (UniProt: A0A0J4U551)
D: 56 - 97 (PDB: 5NSS)
D: 99 - 1407 (UniProt: P0A8T7)
F, G, J, K, L, N: 1 - 275 (UniProt: P37344)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
M: 8 - 49 (PDB: 5NSS)
M: 120 - 258 (UniProt: A0A0J4U551)
M: 259 - 304 (PDB: 5NSS)
M: 306 - 396 (UniProt: A0A1W1PRD8)
M: 398 - 413 (PDB: 5NSS)
M: 415 - 477 (UniProt: A0A0J4U551)
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 14 |
Total formula weight | 693938.9 | |
All* | Total formula weight | 693938.9 |